Multiplex PCR-SSCP analysis for the determination of the ABO genotypes.

(A) The SSCP banding patterns for homozygotes (for O1, O^1v, A1, A^1v, and B) and heterozygotes (for less common alleles A2 and O2). (B) Schematic drawing showing the standard SSCP banding patterns for the 7 common ABO alleles, together with their designations. Note that the homozygous patterns for A2 and O2 are deduced from the heterozygous patterns. For alleles B and O2, the weakly stained fastest-moving band of the F1 fragment overlaps with the strongly stained slowest-moving band of the F3 fragment marked by asterisk (*). (C) The SSCP banding patterns for representative samples containing rare alleles, together with some common phenotypes (O¹O^1v and A¹A2) for comparison. The rare alleles are designated as A^v1, and O^v1 to O^v7.