Quantitative reverse transcription-polymerase chain reaction (RT-PCR) of CO.1 and CO.2 mRNA species.

The experiment was performed on reticulocyte mRNA obtained from the heterozygous individual B2 in family B. In the top panel is an autoradiograph showing the normal bands and the bands expected with CO.1 and CO.2. Longer exposure revealed the 3 specific bands at all the time points. The bottom panel shows phosphorimaging measurements of both the total CO mRNA levels compared with the normal 4.1R mRNA produced in trans (curve) and the relative amounts of CO.1 and CO.2 (bars).