Expression of GATA-1-transfected M1 cells.
(A) Expression of GATA-1 in M1-V1, GATA-1-transfected M1 (M1-G1, -G2, -G3, and -G4), and a human erythrocytic cell line in Northern blot (left panel) and Western blot (right panel) analyses. Total cellular RNA was isolated from each clone, and 15 μg of each sample was electrophoresed on formaldehyde agarose gels. The filter was hybridized with 32P-labeled full length of human GATA-1 complementary DNA or CHO-B (left panel). Total cellular lysates (15 μg per each lane) were subjected to SDS-PAGE and probed with rabbit anti-GATA-1 polyclonal antibody. Immunoreactive proteins were visualized with the enhanced chemiluminescence detection system (right panel). (B) Expression of c-Mpl and CD61 in M1-V1, M1-G1, and M1-G2. Expression of c-Mpl and CD61 was examined by staining with rabbit anti-c-Mpl antiserum or CD61 monoclonal antibody (—) with a reference to rabbit preimmune serum or control antibody of the same isotype (---).
