Up-regulation of EPCR mRNA levels in mice by lipopolysaccharide B from Escherichia coli O127:B8 but not by TNF-.

Mice were injected intravenously with TNF-α (3.6 mg/kg of body weight) or intraperitoneally with lipopolysaccharide B from E coli O127:B8 (LPS) endotoxin (20 mg/kg). RNA was obtained from the heart and lungs at the times indicated (hours after administration of LPS or TNF-α) and analyzed by Northern blotting. The ratios of EPCR to CHO-B are indicated below the blots for each time point. The blots at the top represent the experiments with TNF-α; those at the bottom represent the experiments with endotoxin.