Dose response of GCP-2–induced downmodulation of CXCR2.

CXCR2-expressing HEK 293 cells were incubated with various concentrations of GCP-2 for 2 hours at 37°C. The cells were washed and stained with anti-CXCR2–specific antibodies, and subjected to fluorescence-activated cell sorting (FACS) analysis as described in “Materials and methods.” Each value represents the mean ± SD of 5 independent experiments.