Fluorescence flow cytometry analysis of apoptotic mast cells.

(A) PKCβ-deficient and wild-type mast cells were incubated with IL-3 (upper panels) or without IL-3 for 18 hours and stained with annexin V and 7-amino actinomycin D (7AAD). The left-hand column shows the forward- and side-scatter analysis of the cells. The right-hand column shows a 2-dimensional representation of cells derived from the left-hand column by using annexin V and 7AAD. (B) Graph based on results obtained by fluorescence-activated cell separation analysis done in a manner similar to the analysis shown in Figure 2A for PKCβ-deficient mast cells compared with wild-type cells after growth factor deprivation. (C) A similar graph for cells treated with mitomycin C. Here, the cells were grown with only 20 U of IL-3.