Reactivity of KKO to hPF4/GAG complexes.

Binding of KKO to PF4 and GAGs was assayed by ELISA using wells coated with a fixed concentration of hPF4 (10 μg/mL) and the indicated concentrations of chondroitin sulfate A (ChSO₄A), chondroitin sulfate B (ChSO₄B), chondroitin sulfate C (ChSO₄C), dextran sulfate (Dex- SO₄), and heparan sulfate (HepSO₄). The data shown represent the mean ± 1 SD of duplicate wells and are representative of 2 independent measurements.