Higher expression of both X4-tropic and R5-tropic HIV-1 strains in Der p 1– and SK-specific short-term CD4⁺ T cell lines.

Intracytofluorimetric analysis of IL-4 and IFN-γ production (A and D) and cytofluorimetric detection of CXCR4 and CCR5 surface expression (B and E) by Der p 1– and SK-specific CD4⁺ T cells from 2 different atopic donors, examined on day 15 of culture, as described in “Materials and Methods.” Spontaneous release of p24 Ag on day 21 (day 6 after infection with the X4–HIV-1IIIB or R5-HIV-1BaL strains) by Der p 1–specific (open bars) and SK-specific (black bars) CD4+ T cells (C and F).