Fig. 3.
Kinetics of p24 Ag and β-chemokine production by Th1-polarized or Th2-polarized naive T cells infected with HIV-1BaL.
Spontaneous release of p24 Ag and β-chemokines was assessed in the same supernatants of naive activated CD4+ T cells infected with HIV-1BaL 3 days after primary (A and C) or secondary (B and D) stimulation. Open and closed columns represent p24 Ag concentrations found in supernatants from Th2-polarized or Th1-polarized T cells, respectively. White and gray columns represent concentrations of MIP-1α (hatched areas), MIP-1β (dotted areas), and RANTES (empty areas). Results are expressed as net production of p24 Ag or β-chemokines at the indicated days according to the following formula: concentration at timex − concentration at timex−1. Mean values ± SE of 12 separate experiments (A and C) and 8 separate experiments (B and D) are reported.