Intracellular detection of MIP-1 production by IFN-γ– producing Ag-specific CD4⁺ T cells but not by IL-4–producing antigen-specific CD4⁺ T cells.

(A) T-cell blasts from SK-specific or Der p 1–specific short-term T cell lines were stimulated with PMA plus ionomycin on day 6 after infection with HIV-1BaL strain and simultaneously analyzed for MIP-1αb IFN-γ, and IL-4 content, as described in “Materials and Methods.” (B) IL-4 and IFN-γ production in MIP-1α–negative (R1) and MIP-1α–positive (R2) Der p 1–specific CD4⁺ T cells shown in (A). The area of MIP-1α positivity was determined against an isotype-matched antibody.