Adherence by shear flow of normal- or PS-RBCs to immobilized matrix proteins.

Purified BSA, fibronectin, or thrombospondin were immobilized onto glass slides for 1 hour before being exposed to normal (solid bars) or PS-exposing (shaded bars) RBCs at 1 dyne/cm² in a dynamic flow assay. RBC adherence is reported as a ratio, relative to normal RBC adherence to BSA, which was arbitrarily set to 1. Error bars represent standard error of the means calculated from the absolute values and normalized relative to normal RBC adherence to BSA. *Indicates significant differences between normal- and PS-exposing RBC adherence to BSA (P = .01, n = 5) and between normal- and PS-exposing RBC adherence to TSP (P = .005, n = 5).