Specificity of vMIP-III antibodies and expression of vMIP-III in KS lesions.

(A) Polyclonal antibodies were generated in rabbits and examined for their ability to recognize synthetic or mammalian cell-derived vMIP-III by Western blotting. With the exception of the crude supernatant, 100 ng each sample was loaded. Synthetic vMIP-I and vMIP-II served as negative controls. (B) Expression of vMIP-III in lesions from advanced-stage KS was determined by Western blotting. Blots probed with preimmune sera did not detect these bands.