vMIP-III selectively chemoattracts TH2 cells.

(A) Peripheral blood-derived CD4+ TH1 and TH2 lines were confirmed for their respective lineage designation by intracellular staining for IFN-γ and IL-4 (inset) and tested with lineage-specific chemokines RANTES (TH1), MDC (TH2), and ELC (TH1/2) in chemotaxis assays. Chemokine concentrations were used at concentrations that elicited maximal chemotaxis (10 nmol/L for RANTES and MDC; 5 nmol/L for ELC). (B) TH1 and TH2 lines were examined for their ability to undergo chemotaxis in response to vMIP-III. The chemotactic profile is similar to that for CCR4 transfectants (Figure 4B).