Fig. 3.
HL-60 cell-cell interactions mediated by PMP that was generated by the calcium ionophore A23187.
HL-60 cells (total volume, 6 mL) incubated with PMPs in a calcium-containing buffer were withdrawn at 0.4 dyne/cm2over HL-60 cells prebound to an antihuman CD43 mAb-coated glass slide. The control consisted of washed HL-60 cells with no PMPs. *Indicates that significantly more cell-cell interactions occurred with the addition of PMP (P <.05) compared to control levels. The normalized rate of transient HL-60 cell interactions was determined as in Figure 2. The graph is representative of 5 independent experiments.
