Fig. 4.
HL-60 cell-cell interactions mediated by PMPs require P-selectin and PSGL-1.
HL-60 cells incubated with PMPs generated by calcium ionophore were withdrawn at 0.4 dyne/cm2 over HL-60 cells prebound to an antihuman CD43 mAb-coated slide in the presence of various blocking and nonblocking antibodies. The control consisted of washed HL-60 cells with no PMPs. The normalized rate of transient HL-60 cell interactions was determined as in Figure 2. The graph is representative of 2 independent experiments.
