Neutrophil accumulation on a P-selectin bilayer enhanced by PMP-mediated cell-cell interactions.
Neutrophils were withdrawn over a bilayer containing P-selectin at 1.4 dyne/cm2 in the presence or absence of the anti–L-selectin mAb DREG-56 and at 0.4 dyne/cm2 in the presence or absence of PMPs (0.4 mL) generated by calcium ionophore. *Indicates that the anti–L-selectin antibody significantly reduced neutrophil accumulation on the surface at 5 minutes (P < .01). **Indicates that the addition of PMPs significantly increased neutrophil accumulation at 5 minutes (P < .01). The number of rolling cells on the surface at various time intervals was normalized by assigning the value at 5 minutes for 1.4 dyne/cm2 in the absence of DREG-56 as 100%. Error bars represent ± SEM (n = 3; n = 2 for DREG-56).
