Immunoblotting studies.

Aliquots of 10⁹ washed platelets from an Iy^a-positive individual were lysed, and proteins were separated by using 7.5% SDS-PAGE under nonreducing (n.r.) and reducing (r.) conditions. After proteins were transferred to a nitrocellulose membrane, membrane strips were incubated with anti-Iy^aantiserum (lane 1), MAB SZ2 (lane 2), and MAB Gi27 (lane 3). Antibody binding was detected by using corresponding peroxidase-conjugated antibodies and chemiluminescence substrate.