Fig. 3. Immune precipitation of the Xga and CD99 antigens from transfected RAG cells and erythrocytes. / (A) Intact nontransfected RAG cells or the clone XG13-h.MIC15 were125I-labeled and incubated with NBL-1 or 12E7 antibodies, as noted below the autoradiograph. Solubilized complexes were precipitated, separated on SDS-PAGE, and autoradiographed. (B) Erythrocyte surface proteins from Xg(a+) (female), Xg(a−) (male, CD99-high expressor) or Xg(a−) (female, CD99-low expressor) donors were 125I-labeled. Cell membranes were prepared, incubated with NBL-1 (or the anti-Xga serum Alo.) or 12E7 antibodies, and solubilized complexes were treated as in A. The size (kd) of the detected bands was determined using rainbow-colored protein molecular weight markers (Amersham).
Fig. 3.

Immune precipitation of the Xga and CD99 antigens from transfected RAG cells and erythrocytes.

(A) Intact nontransfected RAG cells or the clone XG13-h.MIC15 were125I-labeled and incubated with NBL-1 or 12E7 antibodies, as noted below the autoradiograph. Solubilized complexes were precipitated, separated on SDS-PAGE, and autoradiographed. (B) Erythrocyte surface proteins from Xg(a+) (female), Xg(a−) (male, CD99-high expressor) or Xg(a−) (female, CD99-low expressor) donors were 125I-labeled. Cell membranes were prepared, incubated with NBL-1 (or the anti-Xga serum Alo.) or 12E7 antibodies, and solubilized complexes were treated as in A. The size (kd) of the detected bands was determined using rainbow-colored protein molecular weight markers (Amersham).

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal