The binding of ARL antibodies to HIV-infected and uninfected lymphocytes.

Human antibody 71-31 (IgG-λ) was used as a positive control to identify HIV-infected cells. F(ab′) fragment specific for human IgG/IgM (H&L) biotin-labeled was used as the secondary reagent, and streptavidin-FITC was used as the tertiary reagent. X axis represents the intensity of fluorescence and Y axis the number of events. Panel A shows binding of 71-31, ARL-7, and ARL-14 antibodies to the cytoplasmic (71-31) and surface (ARL-7 and ARL-14) antigens of infected cells (thick gray line) in comparison to isotype control (thin line). Panel B shows binding of the same antibodies to uninfected cells. Panel C shows binding of ARL-7 and ARL-14 antibodies to K-562, an erytholeukemia cell line, and REH, a pro-B cell line, respectively (thick gray line), in comparison to isotype controls (thin line).