The binding of ARL-7 to surface antigens in relation to cell cycle.

REH cells were first incubated with ARL-7 antibody or isotype control, then fixed, permeabilized, and stained with propidium iodide (PI) before analysis. The upper dot blot shows the nuclear staining with PI (X axis) versus surface staining with isotype-FITC (Y axis), and the lower dot blot shows PI nuclear staining versus ARL-7-FITC surface staining. The cell cycle phases are shown as G0-1(gap 0-1), S (synthesis) and G2-M (gap 2-mitosis).