![Fig. 2. (A) The relative Cl− conductance for normal erythrocytes versus the concentration of NS1652. / The solid line represents the best fit to a single-site inhibitor equation with IC50 = 0.62 μM, and an insensitive fraction (GCl([∞])) of 0.066. (B) Effect of NS1652 on valinomycin-induced erythrocyte dehydration. Erythrocytes were suspended at a cytocrit of 10% and incubated with various concentrations of NS1652 or 4,4′-diisothiocyano-2,2′-stilbene-disulfonic acid (DIDS) for 2 minutes before addition of 5 ∗ 10−8 mol/L valinomycin. After 5 minutes of valinomycin incubation, the cytocrits were measured. The solid line represents the best fit of the NS1652 data (⧫) to a single-site inhibitor equation. The IC50value was estimated at 1.3 μM, the relative volume after valinomycin incubation in absence of blocker (V[0]) was 74.2%, and the relative volume at saturating concentrations of NS1652 (V[∞] + V[0]) was 93.1%. DIDS data (□) are shown for comparison only.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/5/10.1182_blood.v95.5.1842.005a37_1842_1848/6/bloo00537002x.jpeg?Expires=1735828275&Signature=3sggW9OfAXC61nmtobyT9fb~bHRuUIXBpCetaWhsHswUD38~NUxIWy-aiQdxYJCjTtnNdhpYoocHkQJvPYZXrRqi4A0jSkK6S54H00WeMvg4rRG65MDn0iFNStzaHRIWck2DFZtSBKWqHKEiGSL~Pmga~r7tJCamakXtgBbgRRggn3ZY7MnNwoBuB~wqV-tRDITYYIqKDv5eHWSHkaCuwAJcbyDiy1U0Okh1bo~u3Z5SLIU9ukJ~mo77JbbJ57THHZ6Gl9x-ZdjNL07~sV11gubOc7Lm3xu3nv4b9d~YeBVb5jDT4lSZ69T1mGmISooXOcvrJC~Je1Y7ieyl7V0uIA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
(A) The relative Cl− conductance for normal erythrocytes versus the concentration of NS1652.
The solid line represents the best fit to a single-site inhibitor equation with IC50 = 0.62 μM, and an insensitive fraction (GCl([∞])) of 0.066. (B) Effect of NS1652 on valinomycin-induced erythrocyte dehydration. Erythrocytes were suspended at a cytocrit of 10% and incubated with various concentrations of NS1652 or 4,4′-diisothiocyano-2,2′-stilbene-disulfonic acid (DIDS) for 2 minutes before addition of 5 ∗ 10−8 mol/L valinomycin. After 5 minutes of valinomycin incubation, the cytocrits were measured. The solid line represents the best fit of the NS1652 data (⧫) to a single-site inhibitor equation. The IC50value was estimated at 1.3 μM, the relative volume after valinomycin incubation in absence of blocker (V[0]) was 74.2%, and the relative volume at saturating concentrations of NS1652 (V[∞] + V[0]) was 93.1%. DIDS data (□) are shown for comparison only.
