Inhibition of Cl⁻ conductance in sickle (S/S) erythrocytes by NS1652.

Dose-response curves showing the effects of NS1652 on the Cl⁻ conductance in deoxygenated (▪) as well as oxygenated (⧫) sickle cells. A suspension of erythrocytes (cvf = 30%) was deoxygenated in a humidified argon atmosphere for 2 hours. The cells were then packed and stored on ice in a tightly sealed argon-filled vial until use; 100 μL deoxygenated, packed cells were quickly transferred to 3 mL deoxygenated experimental salt solution. The suspension was kept under argon throughout the experiment. The oxygenated cells were handled in parallel, but exposed to the normal atmosphere. Experimental details are otherwise as in Figure 1.