Immunogold Electron Microscopic assays (performed as described before)9. / (A) Ultrathin frozen sections of rat platelets immunolabeled with antibodies to ERK (magnification ×45 000). (B) Well-developed demarcation membranes in a rat (Wistar), TPO-treated megakaryocyte79 immunostained with an antibody to Mst1 (magnification ×48 000), or (C) with an antibody to ERK, which recognizes ERK1 and ERK2 (magnification ×65 400). The arrows point to the demarcation membranes. The Mst1 antiserum (gift of Dr Jonathan Chernoff, Fox Chase Cancer Center, Philadelphia, PA) and anti-ERK2 (K-23) (Santa Cruz Biotechnology, Santa Cruz, CA) or TR2, anti-ERK (gift of Michael Weber, University of Virginia Medical School) were diluted 1:10.

Immunogold Electron Microscopic assays (performed as described before)9.

(A) Ultrathin frozen sections of rat platelets immunolabeled with antibodies to ERK (magnification ×45 000). (B) Well-developed demarcation membranes in a rat (Wistar), TPO-treated megakaryocyte7 9 immunostained with an antibody to Mst1 (magnification ×48 000), or (C) with an antibody to ERK, which recognizes ERK1 and ERK2 (magnification ×65 400). The arrows point to the demarcation membranes. The Mst1 antiserum (gift of Dr Jonathan Chernoff, Fox Chase Cancer Center, Philadelphia, PA) and anti-ERK2 (K-23) (Santa Cruz Biotechnology, Santa Cruz, CA) or TR2, anti-ERK (gift of Michael Weber, University of Virginia Medical School) were diluted 1:10.

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