American Society of Hematology

Fig. 5.

$Fig. 5. Kinetics of representation of HIV-specific and heterologous CTL clones in patient 0407 following treatment of primary HIV infection with HAART. / Sequential cDNA samples from patient 0407 were amplified with the use of cognate TCRBV-specific primers, and fractionated on HMA gels, with cDNAs derived from CTL clones used as controls. These were probed with (A) sense and antisense carrier-specific and (B) clonotype-specific probes, as described in “Materials and methods.” Homoduplex (H) and heteroduplex (h) regions are shown. Heterocomplexes corresponding to specific clonotypes are indicated by arrows. (C) Computerized densitometric analysis of the HMA autoradiograms shown in panel B. The y-axis corresponds to the area under the densitometric profile measured over the heteroduplex region (h) shown in panel B.$

Kinetics of representation of HIV-specific and heterologous CTL clones in patient 0407 following treatment of primary HIV infection with HAART.

Sequential cDNA samples from patient 0407 were amplified with the use of cognate TCRBV-specific primers, and fractionated on HMA gels, with cDNAs derived from CTL clones used as controls. These were probed with (A) sense and antisense carrier-specific and (B) clonotype-specific probes, as described in “Materials and methods.” Homoduplex (H) and heteroduplex (h) regions are shown. Heterocomplexes corresponding to specific clonotypes are indicated by arrows. (C) Computerized densitometric analysis of the HMA autoradiograms shown in panel B. The y-axis corresponds to the area under the densitometric profile measured over the heteroduplex region (h) shown in panel B.

This Feature Is Available To Subscribers Only