Schematic diagram of GFP-tagged CEP110 constructs.

Various regions of CEP110 were tagged with GFP at their C-terminus, transfected, and examined for their ability to target the centrosome. Colocalization of GFP with pericentrin staining was scored asyes, whereas no colocalization was scored as no. The amino acid positions of CEP110 in each construct are shown. Shaded ovals indicate the GFP portion of the fusion protein.