Immunolocalization of endogenous CEP110 and CEP110-FGFR1 fusion protein.

Cos-1 cells transfected with the expression vector pcDNA3, either empty (A, C) or containing CEP110-FGFR1 fusion cDNA (B, D), were subjected to double- immunofluorescence staining with anti-CEP110 antibodies (Ab5′2 and human immune serum), revealed by Alexa-conjugated antirabbit (for Ab5′2) or FITC-conjugated antihuman (for human serum) secondary antibodies and 7-AAD to visualize endogenous CEP110 (arrowheads) and CEP110-FGFR1 (both in green) and the DNA (in red), respectively. Magnification, × 1000.