FISH analysis for chromosomal localization of murinesurvivin locus.

Lymphocytes were isolated from mouse spleen and cultured as detailed in “Materials and Methods.” A biotin-labeled PAC DNA clone containing the entire survivin gene was hybridized with metaphase lymphocyte chromosomes. FISH signals and the DAPI banding pattern were recorded separately, and the assignment of FISH mapping data with chromosomal bands was achieved by superimposing FISH signals with DAPI-banded chromosomes. In the left panel, dots represent the specific FISH signals detected on chromosome 11. The right panel shows the same mitotic figure, stained with DAPI to identify mouse chromosome 11.