Tissue distribution of murine survivin mRNAs.

RT-PCR was performed on mRNA derived from adult tissues using primers that flanked the specific survivin mRNAs. (A) Accumulation of mRNA for survivin₁₄₀ was detected in thymus and testis (primer pair srv86 and srvas311 results in amplicon of 254 bp). Positive controls using cDNA clones 5.20 and 5.25 (see Figure 2) as target DNA, are in 2 lanes at the right side, demonstrating prominent 144 bp and 254 bp bands, respectively. (B) Survivin₁₂₁ mRNA was detected in all tissues (primer pair srv86 and srvas6380 results in amplicon of 332 bp). Survivin₄₀ mRNA using primer pairs srv86 and srvas311, or srv86 and srvas6380 (expecting amplicons of 144 bp or 222 bp, respectively) was not detectable. (C) HPRT transcripts were present in all tissues (221 bp). The right lane in panels B and C are negative controls without target DNA.