Immunoprecipitation of murine survivin from embryos.

Lysates of 12.5 dpc embyros were biotinylated and immunoprecipitated with anti-survivin antibodies (lane 1) or nonspecific antibodies (lane 2). Following separation by SDS-PAGE and transfer to nitrocellulose membranes, filters were incubated with streptavidin-HRP for detection with the ECL kit (Amersham), as detailed in “Materials and Methods.” Molecular weight markers are on the left. Bands representing survivin₁₄₀ and survivin₁₂₁ are visualized at approximately 16 kd and 14 kd, respectively.