Analysis of surface FV expression elicited by various agonists.

Platelets were stimulated with various agonists at the concentrations depicted on the abscissa. In Panel A single agonists were used similar to experiments in Figures 1A and 1B; the percentage of cells with surface FV is shown on the ordinate (mean ± 1 SD; n = 3-8). In Panel B, dual agonist stimulation was performed with thrombin held constant at 5 nmol/L and convulxin varied from 0.5 to 500 ng/mL. Two parameters are reported: COAT-FV and low-level FV corresponding to region M2 and region M1 minus M2, respectively, of Figure 2. Note that in Panel B the percentage of cells with low-level binding remains relatively constant, whereas the number of cells with COAT-FV increases with increasing convulxin concentration (n = 3-12).