Annexin-V binding to platelets stimulated with single or dual agonists.

For Panel A, platelets were activated with the various agonists indicated on the abscissa, and the binding of phycoerythrin-labeled annexin-V was monitored. All convulxin concentrations represent ng/mL and T indicates thrombin at 5 nmol/L. Bars represent mean ± 1 SD, n = 6. Panel B demonstrates the percentage of platelets positive for COAT-FV (abscissa) and annexin-V (ordinate) on stimulation with 5 nmol/L thrombin and the convulxin concentration depicted in the plot. Data are extracted from Figures 3B and 6A. Panel C represents dual labeling of platelets stimulated with convulxin plus thrombin as described in “Materials and methods.” The abscissa (FL₃) depicts biotin-HFV-237/streptavidin TRI-COLOR binding, and the ordinate (FL₂) represents PE–annexin-V binding. Events in region R1 are positive for both annexin-V and FV.