Time course of -granule FV surface expression and microparticle generation.

A time course for the generation of total FV-positive (region M1, Figure 2D) and COAT-FV (region M2, Figure 2D) cells after stimulation with 5 nmol/L thrombin plus 500 ng/mL convulxin is shown in Panel A. Both populations are essentially stable after 3 minutes of activation (mean ± 1 SD; n = 3). For Panel B, the percentage of MP after 2 to 20 minutes of stimulation was determined as detailed in “Materials and methods.” With 2 μmol/L A23187, there is a time-dependent increase in the percentage of MP, whereas the absolute number of MP and their time-dependent increase with 5 nmol/L thrombin plus 500 ng/mL convulxin stimulation is considerably less.