American Society of Hematology

Fig. 10.

$Fig. 10. Contribution of microparticles to prothrombinase activity. / Platelets were activated with either 2 μmol/L A23187 or 5 nmol/L thrombin plus 500 ng/mL convulxin. At various times of platelet activation, samples were briefly centrifuged to pellet intact platelets and leave MP in the supernatant as described in “Materials and methods.” The prothrombinase activity of complete (Panel A) and microparticle-enriched supernatant (Panel B) samples was then determined. Prothrombinase rates for Panel A were normalized to the value for A23187 at 10 minutes (mean ± 1 SD; n = 3). For Panel B, the MP-related prothrombinase activity is presented as percentage of activity for the corresponding unfractionated sample.$

Contribution of microparticles to prothrombinase activity.

Platelets were activated with either 2 μmol/L A23187 or 5 nmol/L thrombin plus 500 ng/mL convulxin. At various times of platelet activation, samples were briefly centrifuged to pellet intact platelets and leave MP in the supernatant as described in “Materials and methods.” The prothrombinase activity of complete (Panel A) and microparticle-enriched supernatant (Panel B) samples was then determined. Prothrombinase rates for Panel A were normalized to the value for A23187 at 10 minutes (mean ± 1 SD; n = 3). For Panel B, the MP-related prothrombinase activity is presented as percentage of activity for the corresponding unfractionated sample.

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