The effect of simultaneous administration of mCTLA4-Ig and factor VIII.

Hemophilia A mice treated as described for Figure 4, with 6 injections of both factor VIII and mCTLA4-Ig, were subsequently given 6 intravenous injections of 0.2 μg of factor VIII at 3-week intervals without additional mCTLA4-Ig (•). Control mice with no prior factor VIII exposure were immunized in parallel (○). Serum samples for the antifactor VIII assay were obtained 3 weeks after the second and sixth injections.