Bone marrow stroma-derived cell lines show differing levels of infectivity with L donovani amastigotes.

(A) Bone marrow stroma-derived cell lines were infected with amastigotes at a multiplicity of infection of 50:1 (open bars), 25:1 (hatched bars), 10:1 (cross-hatched bars), or 5:1 (solid bars). At 1 hour after infection, cells were washed and cytospun onto glass slides. (B) Bone marrow stroma-derived cell lines were infected with amastigotes at a ratio of 25:1. At 1 hour after infection, cells were washed and cytospun onto slides (open bars) or incubated for a further 72 hours (hatched bars). The number of intracellular (IC) parasites per 100 cell nuclei was determined following fixation and Giemsa staining. Data represent the mean ± SEM for triplicate samples at each infection ratio and time point and are representative of 2 independent experiments.