Stromal macrophages do not affect hematopoietic colony formation in the presence of exogenous growth factors.

14M1.4 cells were either untreated (open circles) or infected with amastigotes at a ratio of 25:1 (closed circles), and were overlaid with spleen (A and B) or bone marrow (C-E) cells suspended in methylcellulose containing PWM-SCCM, Epo, and SCF. After 7 days, mature colonies were scored as CFU-GM (A and C), BFU-E (B and D) and CFU-GEMM (E). Data represent the mean ± SEM for triplicate wells and are representative of 2 independent experiments.