L donovani infection of 14M1.4 cells promotes CFU-GM formation in the absence of exogenous growth factors.

14M1.4 cells were untreated (open circles) or infected with amastigotes at a ratio of 25:1 (closed circles) and allowed to adhere before addition of either spleen (A and B) or bone marrow (C-E) cells suspended in methylcellulose, in the absence of growth factors. After 7 days, mature colonies were scored as CFU-GM (A and C), BFU-E (B and D), or CFU-GEMM (E). Data represent the mean ± SEM for triplicate wells and are representative of 2 independent experiments. Significant statistical differences between naı̈ve and infected 14M1.4 cells of P < 0.02 (*) are indicated.