L donovani infection of 14M1.4 cells induces selective accumulation of cytokine/chemokine mRNA.

14M1.4 cells were untreated (A) or infected with amastigotes (B) for 1 hour at 37°C. Infected and naive samples were then washed and resuspended in TRI-reagent in preparation for RNA extraction, or incubated for up to 12 hours before resuspension in TRI-reagent. mRNA accumulation was measured by RT-PCR. Data illustrate PCR products visualized by Southern blotting and enhanced chemiluminescence detection. Bands represent PCR products from 3 individual samples at 2 hours, 6 hours, and 12 hours after infection.