Kinetics of mRNA accumulation following L donovani infection of 14M1.4 cells.

14M1.4 cells were untreated (open circles) or infected with amastigotes (closed circles) at 25:1 for 1 hour at 37°C (as indicated by the horizontal line). mRNA accumulation was measured by RT-PCR at various times after infection and results are expressed in arbitrary densitometry units, normalized for levels of expression of HPRT. Data represent the mean ± SEM for 4 samples per group, from 2 independent experiments. Statistically significant differences between naive and L donovani-infected groups ofP < 0.05 (*) and P < 0.005(**) are indicated.