Expression of recombinant idiotype in stably transfected LCL.

Lymphoma-derived immunoglobulin genes were inserted with an attached hexahistidine tag into the EBV-replicon vector pCEP4. Well-proliferating LCL were electroporated and selected with hygromycin. Hygromycin-resistant LCL were lysed and analyzed by Western blot with an antihistidine antibody. Bound antibody was detected by chemiluminescence. rec Fab, recombinant Fab fragment loaded as control; Fd1, IgL1, LCL transfected with pCEP4 vectors encoding the heavy- and light-chain idiotype gene of patient 1; Fd2, IgL2, LCL transfected with pCEP4 vectors encoding the heavy- and light-chain idiotype gene of patient 2; SM, 10- to 250-kd molecular weight marker.