American Society of Hematology

Fig. 3.

$Fig. 3. HPLC chromatograms demonstrating the subcellular distribution of LTC4 synthase activity in CML CD16+ neutrophils. / CD16+ neutrophils from CML patients were subjected to subcellular fractionation as described in Materials and Methods. Cytosolic (upper panel) and microsomal (lower panel) fractions were incubated with LTA4 (60 μmol/L) and glutathione (5 mmol/L) in the presence of 0.05% BSA at 20°C for 10 minutes. Absorbance was measured at 280 nm. The retention times of LT standards are indicated. One representative experiment out of 4 is shown.$

HPLC chromatograms demonstrating the subcellular distribution of LTC₄ synthase activity in CML CD16⁺ neutrophils.

CD16⁺ neutrophils from CML patients were subjected to subcellular fractionation as described in Materials and Methods. Cytosolic (upper panel) and microsomal (lower panel) fractions were incubated with LTA₄ (60 μmol/L) and glutathione (5 mmol/L) in the presence of 0.05% BSA at 20°C for 10 minutes. Absorbance was measured at 280 nm. The retention times of LT standards are indicated. One representative experiment out of 4 is shown.

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