American Society of Hematology

Fig. 5.

$Fig. 5. Expression of LTC4-producing enzymes in normal and CML CD16+ neutrophils. / Microsomal fractions of CML CD16+ neutrophils, normal CD16+ neutrophils, normal CD16−eosinophil-enriched cell preparations (20 μg protein per sample), and an MGST2 standard obtained from MGST2-transfected Sf9 cells (1 μg protein) were electrophoresed through 14% polyacrylamide gels and electroblotted onto nitrocellulose. Immunoblot analysis was performed using antibodies against LTC4 synthase and MGST2, respectively.$

Expression of LTC₄-producing enzymes in normal and CML CD16⁺ neutrophils.

Microsomal fractions of CML CD16⁺ neutrophils, normal CD16⁺ neutrophils, normal CD16⁻eosinophil-enriched cell preparations (20 μg protein per sample), and an MGST2 standard obtained from MGST2-transfected Sf9 cells (1 μg protein) were electrophoresed through 14% polyacrylamide gels and electroblotted onto nitrocellulose. Immunoblot analysis was performed using antibodies against LTC₄ synthase and MGST2, respectively.

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