Fig. 1.
Chemotactic activity induced by WKYMVm.
Monolayers of KB cells growing on Transwell filters were inverted and transferred to plates with a lower reservoir containing WKYMVm (closed symbols) or fMLF (open symbols). The [51Cr]-labeled neutrophils were added on top of the filters, and transepithelial migration was quantified by measuring the radioactivity accumulating in the lower reservoir after a 2-hour incubation period. Abscissa, agonist concentration; ordinate, transmigration expressed as percentage of cells recovered in the lower compartment. Each symbol represents the mean ± SD of 3 experiments and the values obtained using 10−8 mol/L of the peptides differ significantly (P < .05; paired Studentt test).