![Fig. 7. Changes in cytosolic calcium in undifferentiated HL-60 cells transfected with either the FPR or the lipoxin A4receptor (LXA4R/FPRL1). / Undifferentiated HL-60 cells expressing either FPR or LXA4R/FPRL1 were loaded with 2 μmol Fura-2 and analyzed with respect to the rise in intracellular [Ca++] mediated by the application of peptides. (A) FPR-expressing HL-60 cells have a resting value [Ca++]i around 75 nmol/L. A concentration of [Ca++]i around 150 nmol/L was reached 15 to 20 seconds after addition of fMLFK (final concentration, 10−7 mol/L; open circles) or WKYMVm (final concentration, 10−7 mol/L; closed circles).Inset: The [Ca++]i increase (peak value in percent of maximal value) induced by different concentrations of fMLFK (open circles) and WKYMVm (closed circles), respectively. (B) HL-60 cells expressing LXA4R/FPRL1 have a resting [Ca++]i around 75 nmol/L. A concentration of [Ca++]i around 300 nmol/L was reached 15 to 20 seconds after addition of fMLFK (final concentration, 10−6 mol/L; open circles) or WKYMVm (final concentration, 10−9 mol/L; closed circles).Inset: The [Ca++]i increase (peak value in percent of maximal value) induced by different concentrations of fMLFK (open circles) and WKYMVm (closed circles), respectively. For both figures, abscissa, time of study; ordinate, concentration of [Ca++]i.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/5/10.1182_blood.v95.5.1810.005k06_1810_1818/6/bloo00506007bx.jpeg?Expires=1735559541&Signature=HPPiKb8GEWSKW7vUTYPcrMRhr4EOvl1jmDAXT2lo-wL-wwG-aCXzqfSoXOavGZjJPT-Sjb9A2SWy4J8-z5hRp5Ts80Z11FjGTFE7O77gFAhK6n5vZnjKeS3ZttPpscwowShE4trGSgKyhlvVdAtygqbLn3QtnI5WxpVhh-ZA1YQ~X2-cNs1ndqLGK-E9tCNw8KYQo7FiipsDD3fFeQjHLdmTZX~iO8PyLFeqvWT~tKpH-iHHg~pLtxFvNDhfAJGUgEcxDlKbBs8-f1OeDNNMhnKtRYE-k0nY-HK1WmC5omcSFBC2SBFDNBg44X2gGdAMNTPaWPzrDFbsXU7bnNb4Hw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Changes in cytosolic calcium in undifferentiated HL-60 cells transfected with either the FPR or the lipoxin A4receptor (LXA4R/FPRL1).
Undifferentiated HL-60 cells expressing either FPR or LXA4R/FPRL1 were loaded with 2 μmol Fura-2 and analyzed with respect to the rise in intracellular [Ca++] mediated by the application of peptides. (A) FPR-expressing HL-60 cells have a resting value [Ca++]i around 75 nmol/L. A concentration of [Ca++]i around 150 nmol/L was reached 15 to 20 seconds after addition of fMLFK (final concentration, 10−7 mol/L; open circles) or WKYMVm (final concentration, 10−7 mol/L; closed circles).Inset: The [Ca++]i increase (peak value in percent of maximal value) induced by different concentrations of fMLFK (open circles) and WKYMVm (closed circles), respectively. (B) HL-60 cells expressing LXA4R/FPRL1 have a resting [Ca++]i around 75 nmol/L. A concentration of [Ca++]i around 300 nmol/L was reached 15 to 20 seconds after addition of fMLFK (final concentration, 10−6 mol/L; open circles) or WKYMVm (final concentration, 10−9 mol/L; closed circles).Inset: The [Ca++]i increase (peak value in percent of maximal value) induced by different concentrations of fMLFK (open circles) and WKYMVm (closed circles), respectively. For both figures, abscissa, time of study; ordinate, concentration of [Ca++]i.
