American Society of Hematology

Fig. 8.

$Fig. 8. Antigenic granzyme A ( GrA) and GrA-antithrombin III (ATIII) complexes detected in plasma represent different forms of the protein. Size exclusion chromatography of 750 μL of plasma from a patient with a cytomegalovirus infection containing high levels of antigenic GrA (619 pg/mL) and GrA-ATIII (1.4 ng/mL) complexes. Plasma was eluted under physiologic salt (150 mmol/L) conditions from a 60-mL size exclusion column. Antigenic GrA and GrA-ATIII complexes were detected by enzyme-linked immunosorbent assays as described in “Material and Methods.” As internal markers IgM (Mr 750 kD, fraction 34), IgG (Mr 150 kD, fraction 67), and HSA (Mr 67 KD, fraction 73) were measured.$

Antigenic granzyme A ( GrA) and GrA-antithrombin III (ATIII) complexes detected in plasma represent different forms of the protein. Size exclusion chromatography of 750 μL of plasma from a patient with a cytomegalovirus infection containing high levels of antigenic GrA (619 pg/mL) and GrA-ATIII (1.4 ng/mL) complexes. Plasma was eluted under physiologic salt (150 mmol/L) conditions from a 60-mL size exclusion column. Antigenic GrA and GrA-ATIII complexes were detected by enzyme-linked immunosorbent assays as described in “Material and Methods.” As internal markers IgM (Mr 750 kD, fraction 34), IgG (Mr 150 kD, fraction 67), and HSA (Mr 67 KD, fraction 73) were measured.

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