Fig. 1.
Proliferative activity as determined by 3HTdR uptake assay.
104 cells from LAMA84 and LAMA84R were incubated in 6 replicates in the presence of various concentrations of STI571 ranging from 0.1 to 10 μM. The proliferative assay was performed after 78 hours as described in “Materials and Methods.” The IC50 of the parental line LAMA84 (empty squares) and of the resistant line LAMA84R (black squares) was reached at 0.1 μM and 1 μM, respectively. The total counts per minute (cpm) of untreated samples of LAMA84 and LAMA84R were 161 275 ± 9276 (SD) cpm and 182 961 ± 11 552 (SD) cpm, respectively.
