Fig. 3.
Induction of caspase 3–like activity in LAMA84 and LAMA84R.
The cleavage of the caspase 3 substrate DEVD-amc was evaluated in a fluorimetric assay, as described in “Materials and Methods.” Caspase 3–like activity was detected by reading samples spectrophotofluorimetrically at an excitation wavelength of 380 nm and an emission wavelength of 460 nm.