fMLP-stimulated PMN chemotaxis in gels of collagen (A) and gelatin (B).
PMN, suspended in fMLP 10−9 mol/L, were placed on top of the gels containing fMLP at a concentration of 10−7mol/L. PMN migration into the gel was microscopically quantified after 30 minutes of incubation at 37° C. (A) Effect of antibodies against α2 (mAb P1E6 and mAb AK7), α4 (mAb L25.3), α5 (mAb16), and β1 (mAb13) integrin molecules (20 μg/mL), and of integrin-binding peptides recognizing α2β1 (DGEA, 5 mM), α4β1 (SLIDIP, 100 μM), and α5β1 (RGDGW, 100 μM). The tetrapeptide RGDV (5mM) served as control for DGEA. (B) Effect of antibodies against α2 (mAb AK7) and β1 (mAb13) integrin molecules (20 μg/mL) on fMLP-stimulated PMN migration in gelatin gels. “Unstim” shows PMN migration in respective gel in absence of chemotactic stimulation. Data are based on calculation of migration distance of the leading front and presented as means ± SD of 4 to 6 experiments for each combination analyzed. * indicates significant difference versus fMLP-stimulation alone (P < 0.05).
