Analysis of the backcross mice.
(A) PB-CFC values after 5 days of G-CSF injection. The histograms are the data of 165 males and 95 females bred from D2 and F1 mice and 122 males and 123 females bred from F1 and D2 mice binned by an interval of 0.6 colonies/μL. (B) Linkage analysis of PB-CFC values across the entire genome of both backcross cohorts. The χ2 analysis measures the support for Mendelian inheritance ratios of 1:2:1 at each locus. Only high responders were used for the analysis. The dotted line depicts 32 males and 20 females bred from D2 and F1 mice and 24 males and 24 females bred from F1 and D2 mice. A threshold for suggestive linkage is shown by the dotted line. Chromosomes showing significant or suggestive linkage are indicated. (C) Phenotype of BXD RI strains using 2-3 mice from each strain is plotted as individual histograms. (D) Interval mapping on chromosome 11 using the BXD RI lines. This quantitative trait analysis was performed using the ln PB-CFC values (MapManager QTb19ppc). The threshold for significant linkage is shown by the dotted line.
