Identification of lymphocyte and monocyte subpopulations in cells isolated from WAS and control blood immediately and 24 hours after venipuncture.

(A, C, E) Identification of unlabeled, FITC-anti-CD3–labeled, and PE-anti-CD14–labeled populations in cells isolated from WAS patient blood immediately (A) and 24 hours (C) after venipuncture and in cells isolated from control blood 24 hours (E) after venipuncture. Four different subpopulations were identified in these cells: T lymphocytes (CD3+/CD14-), monocytes (CD14+/CD3-), unlabeled (UN), and necrotic lymphocytes (CD3+/CD14-(N)-seen only in panel C). These graphs are representative of data collected from 2 patients with WAS and at least 5 control donors. (B, D, F) Dot plots showing forward and side-scatter data for cells isolated from WAS patient blood immediately (B) and 24 hours (D) after venipuncture and in cells isolated from control blood 24 hours (F) after venipuncture. Three different subpopulations were identified in these cells: lymphocytes, necrotic lymphocytes (only in C), and monocytes. These graphs are representative of data collected from 2 patients with WAS and at least 5 control donors.